7XZB
Mutant (Y242A) of the N-terminal domain of fucoidan lyase FdlA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 193.15 |
| Detector technology | PIXEL |
| Collection date | 2019-07-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.639, 57.746, 88.202 |
| Unit cell angles | 90.10, 101.33, 92.09 |
Refinement procedure
| Resolution | 27.670 - 2.250 |
| R-factor | 0.1871 |
| Rwork | 0.185 |
| R-free | 0.21980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7xzf |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.892 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.670 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.163 | |
| Rpim | 0.262 | |
| Number of reflections | 46873 | 4394 |
| <I/σ(I)> | 3.3 | |
| Completeness [%] | 95.5 | 96.1 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 291.15 | 0.1 M CAPS pH 10.5, 0.2 M lithium sulfate, 2 M ammonium sulfate |
