7XZ8

Mutant (K141A) of the N-terminal domain of fucoidan lyase FdlA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2021-01-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 61
Unit cell lengths	90.480, 90.480, 123.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.620 - 1.890
R-factor	0.1813
Rwork	0.180
R-free	0.21220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7zxf
RMSD bond length	0.007
RMSD bond angle	0.921
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.470	1.930
High resolution limit [Å]	1.890	1.890
Rmeas	0.063	0.078
Number of reflections	45444	2495
<I/σ(I)>	24.4
Completeness [%]	99.1
Redundancy	19.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	291.15	0.1 M CAPS, pH 10.5, 0.2 M lithium sulfate, 2 M ammonium sulfate