7XYV

Crystal structure of ZYG11B bound to SFLH degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	52.443, 97.072, 122.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.170 - 2.520
R-factor	0.2199
Rwork	0.217
R-free	0.27380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ep1
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.020	2.650
High resolution limit [Å]	2.520	2.520
Rmerge	0.076	1.587
Number of reflections	21863	3133
<I/σ(I)>	18.4
Completeness [%]	99.5
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M MES (pH 6.5), 1.6 M MgSO4