7XYU

Crystal structure of ZER1 bound to TFLH degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 61
Unit cell lengths	67.779, 67.779, 418.181
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.900 - 2.700
R-factor	0.2508
Rwork	0.248
R-free	0.30370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ep3
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.050	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.016	0.120
Number of reflections	29654	2990
<I/σ(I)>	33.3
Completeness [%]	99.9
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.16 M Sodium Malonate pH 7.0, 20% PEG 3350