7XYS

Crystal structure of ZER1 bound to SFLH degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-06
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.07
Spacegroup name	P 1 21 1
Unit cell lengths	64.137, 137.191, 69.484
Unit cell angles	90.00, 117.50, 90.00

Refinement procedure

Resolution	36.720 - 1.700
R-factor	0.1626
Rwork	0.147
R-free	0.18340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ep3
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.530	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.096	0.453
Number of reflections	116008	36520
<I/σ(I)>	13.33
Completeness [%]	99.5
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M NH4F, 20% PEG3350