7XYR

Cystal Structure of Beta-glucuronidase from Bacteroides thetaiotaomicron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-09
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	2.0
Spacegroup name	C 2 2 21
Unit cell lengths	101.582, 122.249, 105.122
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.892 - 2.000
Rwork	0.190
R-free	0.23220
Structure solution method	AB INITIO PHASING
RMSD bond length	0.009
RMSD bond angle	1.502
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.892	2.071
High resolution limit [Å]	2.000	2.000
Number of reflections	44460	4387
<I/σ(I)>	12.71
Completeness [%]	99.6
Redundancy	2
CC(1/2)	0.997	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	10%PEG3350, 0.2 M NaCl,0.1 M MES PH 5.5