7XYO

Crystal Structure of a M61 aminopeptidase family member from Myxococcus fulvus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	96.679, 113.278, 132.345
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.110 - 2.700
R-factor	0.2188
Rwork	0.217
R-free	0.24960
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.155	0.066	0.905
Rmeas	0.168	0.072	0.986
Rpim	0.065	0.029	0.386
Total number of observations	266197
Number of reflections	40390	2205	1971
<I/σ(I)>	4.3
Completeness [%]	99.9	99.7	99.6
Redundancy	6.6	6.1	6.4
CC(1/2)		0.996	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289	Sodium acetate, Tris-HCl, PEG10000