7XYE
The apo structure of Orf1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2021-03-24 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 103.694, 108.507, 134.911 |
| Unit cell angles | 90.12, 89.95, 83.75 |
Refinement procedure
| Resolution | 29.669 - 2.482 |
| Rwork | 0.220 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7xqa |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.404 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.560 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.058 | 0.410 |
| Rmeas | 0.066 | 0.465 |
| Rpim | 0.031 | 0.217 |
| Number of reflections | 201246 | 19204 |
| <I/σ(I)> | 23.4 | 3.2 |
| Completeness [%] | 97.5 | 93 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris-HCl pH8.5, 0.2 M KBr, 8 % PEG 550 MME and 8 % PEG 20000 |
