7XY5

HapR_Double Mutant with CHES buffer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-23
Detector	Bruker PHOTON III
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	89.934, 44.773, 53.414
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.707 - 2.370
R-factor	0.2355
Rwork	0.231
R-free	0.27850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pbx
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.280	2.450
High resolution limit [Å]	2.370	2.370
Number of reflections	16867	893
<I/σ(I)>	13.5
Completeness [%]	99.0
Redundancy	7
CC(1/2)	0.990	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	297	7-8% PEG 8000, 100 mM CHES pH 9.5, 200 mM NaCl