7XXX

Macaca mulatta galectin-10/Charcot-Leyden crystal protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97930
Spacegroup name	P 65 2 2
Unit cell lengths	48.589, 48.589, 258.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.490 - 1.940
R-factor	0.1797
Rwork	0.176
R-free	0.21210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xrg
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.490	1.990
High resolution limit [Å]	1.940	1.940
Rmerge	0.095	0.578
Number of reflections	14536	962
<I/σ(I)>	22.6
Completeness [%]	99.9
Redundancy	18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG