7XXV

Macaca fascicularis galectin-10/Charcot-Leyden crystal protein with lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97930
Spacegroup name	P 65 2 2
Unit cell lengths	48.528, 48.528, 256.565
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.970 - 1.600
R-factor	0.1771
Rwork	0.176
R-free	0.19290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xrg
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.970	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.068	0.768
Number of reflections	24980	1152
<I/σ(I)>	26.5
Completeness [%]	99.7
Redundancy	17.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG