7XXS
HapR mutant I141V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-27 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.718, 85.331, 110.622 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.449 - 2.250 |
| R-factor | 0.2375 |
| Rwork | 0.233 |
| R-free | 0.27670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7xxo |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.450 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 134666 | 2033 |
| <I/σ(I)> | 18.4 | |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 6.5 | 6.4 |
| CC(1/2) | 0.999 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 295 | 7% PEG 8000, 100 mM CHES pH 9.5, 200 mM Nacl |
