7XWY

Crystal structure of spFft3 N-terminal truncation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-12-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	51.716, 36.106, 101.973
Unit cell angles	90.00, 95.91, 90.00

Refinement procedure

Resolution	38.132 - 2.250
R-factor	0.1882
Rwork	0.183
R-free	0.23520
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.132	2.310
High resolution limit [Å]	2.250	2.250
Number of reflections	33411	1220
<I/σ(I)>	9.2
Completeness [%]	99.0
Redundancy	6.6
CC(1/2)	0.971	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M Ammonium sulfate, 0.1 M MES monohydrate pH 6.5, 30% w/v Polyethylene glycol monomethyl ether 5,000