7XVV

Structure of neuraminidase from influenza B-like viruses derived from spiny eel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-07
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	I 4
Unit cell lengths	125.890, 125.890, 110.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.630 - 1.850
R-factor	0.1568
Rwork	0.155
R-free	0.18670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xvr
RMSD bond length	0.007
RMSD bond angle	0.918
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.850	1.850
Rpim	0.047	0.403
Number of reflections	61178
<I/σ(I)>	12.7	2
Completeness [%]	83.0
Redundancy	8.8
CC(1/2)	0.998	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	291	4.0 M Potassium formate, 0.1 M BIS-TRIS propane pH 9.0, 2% w/v Polyethylene glycol monomethyl ether 2,000. NA crystals were soaked in 10 mM zanamivir for 30 min