7XVU
Structure of neuraminidase from influenza B-like viruses derived from spiny eel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-07 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | I 4 |
Unit cell lengths | 126.417, 126.417, 110.478 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.590 - 1.600 |
R-factor | 0.1587 |
Rwork | 0.158 |
R-free | 0.18040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7xvr |
RMSD bond length | 0.006 |
RMSD bond angle | 0.914 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rpim | 0.056 | 0.488 |
Number of reflections | 96777 | |
<I/σ(I)> | 11 | 2.4 |
Completeness [%] | 85.3 | 100 |
Redundancy | 7.4 | 7.5 |
CC(1/2) | 0.998 | 0.818 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 4.0 M Potassium formate, 0.1 M BIS-TRIS propane pH 9.0, 2% w/v Polyethylene glycol monomethyl ether 2,000. NA crystals were soaked in 10 mM oseltamivir for 30 min. |