7XVL

Crystal Structure of Nucleosome-H1.0 Linker Histone Assembly (sticky-169an DNA fragment)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98.15
Detector technology	PIXEL
Collection date	2017-09-17
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	213.850, 102.460, 218.289
Unit cell angles	90.00, 100.49, 90.00

Refinement procedure

Resolution	88.439 - 3.506
Rwork	0.206
R-free	0.27950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ut9 4qlc
RMSD bond length	0.006
RMSD bond angle	1.349
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.439	3.570
High resolution limit [Å]	3.506	3.510
Number of reflections	117231	5763
<I/σ(I)>	8	1.1
Completeness [%]	100.0	100
Redundancy	6.1	5.9
CC(1/2)	1.000	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	40-45mM CaCl2, 25mM KCl, 10mM Na-acetate (pH 4.5)