7XVK

Modularity of Phytophthora effectors enables host mimicry of a principal phosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-01-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	76.211, 111.552, 161.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.770 - 2.290
R-factor	0.2093
Rwork	0.209
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gnc 2npp
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.290	4.950	2.300
Rmerge	0.062	0.024	0.640
Rmeas	0.068	0.027	0.696
Rpim	0.027	0.011	0.270
Number of reflections	62511	6597	6142
<I/σ(I)>	8.6
Completeness [%]	99.9	99.8	99.6
Redundancy	6.4	6.2	6.5
CC(1/2)		0.999	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	5% PEG 20000 0.1 M Bis tris pH 5.8 5 mM MgSO4