7XVI

pathogen effectors which are essential to cause plant disease by manipulating cellular processes in the host

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-10-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.987
Spacegroup name	P 2 21 21
Unit cell lengths	47.846, 89.385, 129.367
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.880 - 3.110
R-factor	0.2441
Rwork	0.240
R-free	0.27640
Structure solution method	FOURIER SYNTHESIS
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.260
High resolution limit [Å]	3.110	6.780	3.150
Rmerge	0.096	0.065	0.418
Rmeas	0.109	0.072	0.497
Rpim	0.051	0.030	0.261
Number of reflections	10221	1151	946
<I/σ(I)>	12.1
Completeness [%]	97.3	99.1	91.3
Redundancy	4	5.2	3.3
CC(1/2)		0.992	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	19.5 % PEG 600 0.1 M Bistris propane pH 7.5 1 mM CdCl2