7XVI
pathogen effectors which are essential to cause plant disease by manipulating cellular processes in the host
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-10-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 47.846, 89.385, 129.367 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.880 - 3.110 |
| R-factor | 0.2441 |
| Rwork | 0.240 |
| R-free | 0.27640 |
| Structure solution method | FOURIER SYNTHESIS |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
| High resolution limit [Å] | 3.110 | 6.780 | 3.150 |
| Rmerge | 0.096 | 0.065 | 0.418 |
| Rmeas | 0.109 | 0.072 | 0.497 |
| Rpim | 0.051 | 0.030 | 0.261 |
| Number of reflections | 10221 | 1151 | 946 |
| <I/σ(I)> | 12.1 | ||
| Completeness [%] | 97.3 | 99.1 | 91.3 |
| Redundancy | 4 | 5.2 | 3.3 |
| CC(1/2) | 0.992 | 0.707 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 19.5 % PEG 600 0.1 M Bistris propane pH 7.5 1 mM CdCl2 |
