7XV6

Crystal structure of the Human TR4 DNA-Binding Domain with C-terminal extension (DBD-CTE) Homodimer Bound to DR1 Response Element

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-07
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	52.146, 52.146, 242.701
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.530 - 2.300
Rwork	0.188
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dzu
RMSD bond length	0.010
RMSD bond angle	1.904
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.360
High resolution limit [Å]	2.297	2.297
Number of reflections	15386	12867
<I/σ(I)>	20.2
Completeness [%]	96.3
Redundancy	7.1
CC(1/2)	0.998	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 8000, Tris, ammonium sulfate