7XV4

Crystal structure of RPA70N-ATRIP fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97852
Spacegroup name	P 21 21 21
Unit cell lengths	39.035, 53.110, 55.224
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.280 - 1.600
R-factor	0.1968
Rwork	0.195
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eay
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20rc1_4392)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.280	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.037	0.149
Rpim	0.011	0.043
Number of reflections	29274	2826
<I/σ(I)>	43.83
Completeness [%]	100.0	100
Redundancy	12.6
CC(1/2)	1.000	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	278	2400 mM ammonium sulfate