7XV0

Crystal structure of RPA70N-BLMp1 fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	38.088, 53.644, 54.501
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.230 - 1.500
R-factor	0.1704
Rwork	0.169
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eay
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20rc1_4392)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.230	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.031	0.088
Rpim	0.014	0.038
Number of reflections	33810	2484
<I/σ(I)>	34.45
Completeness [%]	98.6
Redundancy	6.1
CC(1/2)	0.999	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	278	2000 mM Ammonium sulfate