7XTR
The apo structure of the engineered TfCut
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2020-11-21 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.34138 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.689, 41.961, 80.767 |
| Unit cell angles | 90.00, 92.61, 90.00 |
Refinement procedure
| Resolution | 32.680 - 2.200 |
| R-factor | 0.1769 |
| Rwork | 0.171 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cg2 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.200 | 2.230 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.064 | 0.099 |
| Number of reflections | 38580 | 860 |
| <I/σ(I)> | 16.1 | 3 |
| Completeness [%] | 96.5 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 273 | 20% w/v PEG3350, 0.2 M Sodium nitrate, 0.1 M BIS-Tris propane 8.5 |
