7XTN
Crystal structure of the C-terminal domain of Bombyx mori N-acetylglucosaminyltransferase IV in complex with N-acetylglucosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-05 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.697, 64.098, 79.148 |
| Unit cell angles | 90.00, 95.89, 90.00 |
Refinement procedure
| Resolution | 32.545 - 1.150 |
| Rwork | 0.193 |
| R-free | 0.20870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold2 model |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.677 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.370 | 1.210 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.046 | 0.810 |
| Rmeas | 0.050 | 0.879 |
| Rpim | 0.019 | 0.336 |
| Number of reflections | 113597 | 15961 |
| <I/σ(I)> | 18.1 | 2.3 |
| Completeness [%] | 98.6 | 94.9 |
| Redundancy | 6.7 | 6.6 |
| CC(1/2) | 0.999 | 0.782 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M MES-NaOH, pH 7.0, 12%(w/v) PEG20000, 10 mM N-acetylglucosamine |
