7XTM

Crystal structure of the C-terminal domain of Bombyx mori N-acetylglucosaminyltransferase IV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-05
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	32.733, 64.466, 78.810
Unit cell angles	90.00, 95.09, 90.00

Refinement procedure

Resolution	33.547 - 1.470
Rwork	0.224
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold2 model
RMSD bond length	0.012
RMSD bond angle	1.729
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.250	1.550
High resolution limit [Å]	1.470	1.470
Rmerge	0.052	0.526
Rmeas	0.057	0.588
Rpim	0.022	0.255
Number of reflections	53654	6660
<I/σ(I)>	18.2	2.7
Completeness [%]	96.6	83
Redundancy	6.4	5
CC(1/2)	0.999	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M MES-NaOH, pH 7.0, 12%(w/v) PEG20000