7XTL
Crystal structure of the C-terminal domain of human N-acetylglucosaminyltransferase IVa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.699, 32.490, 85.629 |
| Unit cell angles | 90.00, 94.71, 90.00 |
Refinement procedure
| Resolution | 50.579 - 1.972 |
| Rwork | 0.220 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold2 model |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.704 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.340 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.207 | 0.822 |
| Rmeas | 0.216 | 0.855 |
| Rpim | 0.060 | 0.234 |
| Number of reflections | 20101 | 2924 |
| <I/σ(I)> | 8.4 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 13 |
| CC(1/2) | 0.990 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M lithium nitrate, 20%(w/v) PEG3350 |
