7XT1
Crystal structure of Bypass-of-forespore protein C from Bacillus Subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.427, 46.004, 93.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.520 - 1.980 |
| R-factor | 0.2258 |
| Rwork | 0.223 |
| R-free | 0.27270 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.650 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.079 | 1.421 |
| Number of reflections | 10268 | 988 |
| <I/σ(I)> | 30.1 | |
| Completeness [%] | 97.9 | 99.5 |
| Redundancy | 11.8 | |
| CC(1/2) | 0.693 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M sodium acetate trihydrate pH4.5, 30% w/v polyethylene glycol 1500 |
