7XT0
Crystal structure of RNA helicase from Saint Louis encephalitis virus and discovery of its inhibitors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.467, 80.656, 104.761 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.970 - 2.480 |
| R-factor | 0.2065 |
| Rwork | 0.203 |
| R-free | 0.27430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v8o |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.675 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.113 | 0.113 |
| Number of reflections | 38975 | 16033 |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 84.8 | |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289.15 | magnesium chloride hexahydrate, Hepes, pH 7.0, PEG 3350, nickel chloride hexahydrate |
