7XS4

Crystal structure of URT1 in complex with AAAU RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	63.164, 63.264, 81.387
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.257 - 1.846
R-factor	0.1746
Rwork	0.173
R-free	0.20150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l3f
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.880
High resolution limit [Å]	1.846	1.850
Rmerge	0.078
Number of reflections	28694	2803
<I/σ(I)>	24.08
Completeness [%]	100.0
Redundancy	10.6
CC(1/2)	1.000	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M sodium HEPES, pH 7.5, 27% PEG 600