7XS3
AlXyn26A E243A-X3X4X
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-21 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 48.419, 107.051, 118.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.800 - 1.900 |
| R-factor | 0.1731 |
| Rwork | 0.171 |
| R-free | 0.21970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7xjr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.793 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.790 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.055 | 0.229 |
| Number of reflections | 45545 | 2349 |
| <I/σ(I)> | 25.7 | |
| Completeness [%] | 92.4 | |
| Redundancy | 7.7 | |
| CC(1/2) | 0.995 | 0.923 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M sodium chloride, 0.1 M Bis-Tris (pH 5.5), 25% (w/v) PEG 3350 |
