7XRJ

crystal structure of N-acetyltransferase DgcN-25328

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-29
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5406
Spacegroup name	I 2 2 2
Unit cell lengths	57.490, 130.800, 131.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.910 - 2.200
R-factor	0.1838
Rwork	0.182
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2obn
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.360	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.143	0.385
Rmeas	0.159	0.435
Rpim	0.069	0.196
Number of reflections	25305	2118
<I/σ(I)>	11.9	4.4
Completeness [%]	99.0	97.6
Redundancy	4.9	4.3
CC(1/2)	0.980	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M NaCl, 0.1 M Bis-Tris (pH 6.5) and 1.5 M (NH4)2SO4