7XRG
ferritin nanocage assembly with nickel ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL18U |
| Synchrotron site | NFPSS |
| Beamline | BL18U |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2020-11-09 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 117.278, 117.278, 117.278 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.640 - 1.900 |
| R-factor | 0.1928 |
| Rwork | 0.190 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6a4u |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.640 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 21291 | 1833 |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 95.1 | |
| Redundancy | 1 | |
| CC(1/2) | 0.992 | 0.992 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | hepes, sodium chloride |
