7XR1
Crystal structure of T2R-TTL-3a complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.096, 157.528, 181.017 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.350 - 2.810 |
R-factor | 0.2043 |
Rwork | 0.203 |
R-free | 0.24640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i55 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.21) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 118.830 | 118.830 | 2.960 |
High resolution limit [Å] | 2.810 | 8.890 | 2.810 |
Rmerge | 0.312 | 0.249 | 0.756 |
Rmeas | 0.336 | 0.270 | 0.810 |
Rpim | 0.122 | 0.102 | 0.287 |
Total number of observations | 550136 | 16370 | 81622 |
Number of reflections | 73641 | 2533 | 10628 |
<I/σ(I)> | 5.4 | 7.4 | 2.5 |
Completeness [%] | 99.9 | 99.4 | 100 |
Redundancy | 7.5 | 6.5 | 7.7 |
CC(1/2) | 0.956 | 0.945 | 0.825 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7 |