7XQU
The structure of FLA-E*00301/EM-FECV-10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 291 |
| Detector technology | PIXEL |
| Collection date | 2018-01-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 132.221, 132.221, 130.846 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.040 - 2.600 |
| R-factor | 0.2219 |
| Rwork | 0.219 |
| R-free | 0.27570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xmf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.033 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.110 | 0.669 |
| Number of reflections | 35453 | 35453 |
| <I/σ(I)> | 5.909 | |
| Completeness [%] | 97.9 | |
| Redundancy | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 0.1M HEPES sodium, 2% VL Polyethylene glycol 400, 2.0M Ammonium sulfate |
