7XQT

The structure of FLA-K*00701/KP-FECV-11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	53.503, 70.758, 119.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.570 - 2.800
R-factor	0.2288
Rwork	0.225
R-free	0.29330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xfm
RMSD bond length	0.009
RMSD bond angle	1.145
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.840	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.277	1.450
Number of reflections	11706	11706
<I/σ(I)>	12.9
Completeness [%]	99.9
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291.15	0.1M Sodium chloride, 0.1M HEPES PH 7, 25% (W/V) SOKALAN PA 25 CL