7XPQ

Crystal Structure of UDP-Glc/GlcNAc 4-Epimerase with NAD/UDP-GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	65.873, 84.945, 73.436
Unit cell angles	90.00, 110.69, 90.00

Refinement procedure

Resolution	20.077 - 2.150
R-factor	0.1998
Rwork	0.197
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xpo
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.077	2.227
High resolution limit [Å]	2.150	2.150
Number of reflections	40224	3959
<I/σ(I)>	6.42
Completeness [%]	96.7
Redundancy	7
CC(1/2)	0.991	0.197

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	0.1M Sodium malonate pH 5.0, 12% w/V PEG 3350