7XPP
Crystal Structure of UDP-Glc/GlcNAc 4-Epimerase with NAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 163.040, 88.149, 82.431 |
Unit cell angles | 90.00, 117.24, 90.00 |
Refinement procedure
Resolution | 34.990 - 2.600 |
R-factor | 0.2348 |
Rwork | 0.233 |
R-free | 0.26460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7xpo |
RMSD bond length | 0.007 |
RMSD bond angle | 0.907 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.990 | 2.693 |
High resolution limit [Å] | 2.400 | 2.600 |
Number of reflections | 38192 | 3150 |
<I/σ(I)> | 13.36 | |
Completeness [%] | 98.2 | |
Redundancy | 6.8 | |
CC(1/2) | 0.999 | 0.935 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 300 | 0.1M Sodium malonate pH 5.0, 12% w/V PEG 3350 |