7XPO

Crystal Structure of UDP-Glc/GlcNAc 4-Epimerase with NAD/UDP-Glc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	60.933, 84.657, 73.514
Unit cell angles	90.00, 109.32, 90.00

Refinement procedure

Resolution	27.224 - 1.250
R-factor	0.1464
Rwork	0.145
R-free	0.17260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hzj
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.224	1.295
High resolution limit [Å]	1.250	1.250
Number of reflections	191996	18989
<I/σ(I)>	15.81
Completeness [%]	98.9
Redundancy	6.6
CC(1/2)	0.999	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	0.1M Sodium malonate pH 5.0, 12% w/v PEG 3350