7XPK

crystal structure of rice ASI1 BAH domain in complex with a rice SUVH6 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	59.715, 72.939, 76.581
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.291 - 1.800
R-factor	0.2013
Rwork	0.200
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xpj
RMSD bond length	0.003
RMSD bond angle	0.678
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.098	0.837
Number of reflections	31676	2996
<I/σ(I)>	19	1.4
Completeness [%]	99.8	99.9
Redundancy	6.5	6.3
CC(1/2)	1.000	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	30% PEG400 and 0.1 M CHES, pH 9.5