7XP9

Phytophthora infesfans RxLR effector AVRvnt1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 65 2 2
Unit cell lengths	54.004, 54.004, 116.547
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.470 - 1.930
R-factor	0.2263
Rwork	0.222
R-free	0.26290
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.960
High resolution limit [Å]	1.930	5.240	1.930
Rmerge	0.058	0.050	0.165
Rmeas	0.059	0.051	0.167
Rpim	0.010	0.009	0.026
Number of reflections	14032	466	364
<I/σ(I)>	23.6
Completeness [%]	96.9	92.6	95.8
Redundancy	40.2	31.3	41.4
CC(1/2)		0.999	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277.15	25% w/v Polyethylene glycol 3,350, 0.1 M Bis-Tris pH 5.5 and 0.2 M Li2SO4