7XP3

DNA complex form of ORESARA1(ANAC092) NAC Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-05-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 62
Unit cell lengths	92.592, 92.592, 216.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.300 - 3.250
R-factor	0.215
Rwork	0.213
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xlj
RMSD bond length	0.026
RMSD bond angle	1.541
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.300	3.361
High resolution limit [Å]	3.240	3.245
Number of reflections	16649	1663
<I/σ(I)>	18.4	2.18
Completeness [%]	99.5
Redundancy	17
CC(1/2)	0.947	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M potassium sulfate, 20% PEG3350