7XP1

Crystal structure of PmiR from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-11-15
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9793
Spacegroup name	I 41 2 2
Unit cell lengths	74.021, 74.021, 186.543
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.220 - 2.500
R-factor	0.2143
Rwork	0.212
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xp0
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.047	0.030	0.393
Rmeas	0.048	0.031	0.416
Rpim	0.010	0.007	0.127
Total number of observations	159703
Number of reflections	9006	1029	646
<I/σ(I)>	11.4
Completeness [%]	96.4	99.6	71.6
Redundancy	17.7	22.3	8.1
CC(1/2)		1.000	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	290	MES/Sodium hydroxide, Magnesium sulfate