7XOI
Aspergillus sojae alpha-glucosidase AsojAgdL in complex with trehalose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 95.325, 106.036, 206.366 |
| Unit cell angles | 90.58, 90.06, 92.20 |
Refinement procedure
| Resolution | 49.140 - 2.300 |
| R-factor | 0.22912 |
| Rwork | 0.227 |
| R-free | 0.26685 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w38 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.283 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.140 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.071 | 0.239 |
| Rpim | 0.088 | 0.374 |
| Number of reflections | 349142 | 17217 |
| <I/σ(I)> | 5.5 | 1.9 |
| Completeness [%] | 97.3 | 96.6 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 13% polyethylene glycol 8000, 0.2 M ammonium acetate, 0.1 M sodium citrate buffer |
