7XNM

Structure of porcine dipeptidyl peptidase 4 inhibitory peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	190
Detector technology	PIXEL
Collection date	2021-02-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97894
Spacegroup name	C 1 2 1
Unit cell lengths	257.340, 75.617, 121.679
Unit cell angles	90.00, 110.20, 90.00

Refinement procedure

Resolution	29.480 - 3.580
R-factor	0.2289
Rwork	0.223
R-free	0.29890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wcy
RMSD bond length	0.004
RMSD bond angle	0.676
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.480	3.600
High resolution limit [Å]	3.575	3.575
Number of reflections	26023	168262
<I/σ(I)>	2
Completeness [%]	99.0
Redundancy	6.5
CC(1/2)	0.999	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	20% PEG 3350, 0.2 M ammonium chloride