7XNM
Structure of porcine dipeptidyl peptidase 4 inhibitory peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2021-02-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97894 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 257.340, 75.617, 121.679 |
| Unit cell angles | 90.00, 110.20, 90.00 |
Refinement procedure
| Resolution | 29.480 - 3.580 |
| R-factor | 0.2289 |
| Rwork | 0.223 |
| R-free | 0.29890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wcy |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.676 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.480 | 3.600 |
| High resolution limit [Å] | 3.575 | 3.575 |
| Number of reflections | 26023 | 168262 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.999 | 0.717 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 20% PEG 3350, 0.2 M ammonium chloride |
