7XNF

Structure of SARS-CoV-2 antibody P2C-1F11 with GX/P2V/2017 RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	55.722, 85.440, 183.739
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.610 - 2.790
R-factor	0.3225
Rwork	0.320
R-free	0.37950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cdi
RMSD bond length	0.002
RMSD bond angle	0.501
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.790	2.800
Rmerge	0.153	0.925
Number of reflections	22549	2186
<I/σ(I)>	11.905
Completeness [%]	99.8
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M MES monohydrate (pH 6.0), 20% w/v Polyethylene glycol monomethyl ether 2000