7XN2
Crystal structure of CvkR, a novel MerR-type transcriptional regulator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 78.579, 49.703, 40.520 |
Unit cell angles | 90.00, 116.04, 90.00 |
Refinement procedure
Resolution | 24.240 - 1.600 |
R-factor | 0.1953 |
Rwork | 0.194 |
R-free | 0.21810 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
Rmerge | 0.054 | 0.037 | 0.441 |
Rmeas | 0.061 | 0.041 | 0.491 |
Rpim | 0.027 | 0.018 | 0.211 |
Total number of observations | 89996 | ||
Number of reflections | 18581 | 1919 | 1830 |
<I/σ(I)> | 7.8 | ||
Completeness [%] | 99.9 | 99.8 | 100 |
Redundancy | 4.8 | 4.9 | 5 |
CC(1/2) | 0.997 | 0.877 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291.15 | 0.1 M phosphate citrate, 15% PEG300, 10 mM ATP |