7XMH
Crystal structure of a rice class IIIb chitinase, Oschib2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.980 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.698, 69.403, 73.019 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.720 - 1.180 |
Rwork | 0.160 |
R-free | 0.16580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rl3 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.494 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.720 | 1.200 |
High resolution limit [Å] | 1.180 | 1.180 |
Rmerge | 0.087 | 0.167 |
Number of reflections | 101845 | 4567 |
<I/σ(I)> | 32.49 | |
Completeness [%] | 99.5 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | Sodium acetate trihydrate pH 4.5, 3.0 M Sodium chloride |