7XL7

Function And Structure Research of Salmonella typhimurium Unknown Functional Protein MicN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-22
Detector	RAYONIX MX-225
Wavelength(s)	0.9791
Spacegroup name	P 64
Unit cell lengths	74.967, 74.967, 65.405
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.480 - 1.850
R-factor	0.2268
Rwork	0.226
R-free	0.25310
Structure solution method	AB INITIO PHASING
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.480	2.350
High resolution limit [Å]	1.850	1.850
Rmerge	0.074	0.274
Number of reflections	16080	885
<I/σ(I)>	0.83
Completeness [%]	90.4
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	Lithium sulfate monohydrate, Bis-Tris propane pH7.0