7XJC

Crystal structure of bacteriorhodopsin in the ground and K states after green laser irradiation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	15
Detector technology	PIXEL
Collection date	2020-10-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.8
Spacegroup name	P 63
Unit cell lengths	60.570, 60.570, 110.890
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.330
Rwork	0.131
R-free	0.17250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zil
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	SHELX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.420
High resolution limit [Å]	1.330	3.990	1.330
Rmerge	0.074	0.046	1.157
Rmeas	0.082	0.051	1.281
Number of reflections	52175	1994	8408
<I/σ(I)>	11.1	34.98	1.35
Completeness [%]	99.9	99.8	99.8
Redundancy	5.81	5.874	5.507
CC(1/2)	0.998	0.997	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.6	293	MO, 2.0-2.5 M Na/K phosphate pH 5.6