7XIK
SARS-CoV-2-Omicron-RBD and B38-GWP/P-VK antibody complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-01-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.959, 145.859, 144.152 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.410 - 2.890 |
| R-factor | 0.2158 |
| Rwork | 0.211 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7bz5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.743 |
| Data reduction software | DENZO |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.413 | 73.413 | 2.903 |
| High resolution limit [Å] | 2.890 | 13.403 | 2.893 |
| Rmerge | 0.262 | 0.058 | 1.539 |
| Rmeas | 0.272 | 0.061 | 1.597 |
| Rpim | 0.075 | 0.018 | 0.425 |
| Total number of observations | 2250 | 2613 | |
| Number of reflections | 20329 | 227 | 192 |
| <I/σ(I)> | 10.2 | 21.5 | 2.1 |
| Completeness [%] | 99.8 | 92.3 | 100 |
| Redundancy | 13 | 9.9 | 13.6 |
| CC(1/2) | 0.994 | 0.999 | 0.723 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 15% v/v 2-Propanol, 0.1M Sodium citrate tribasic dihydrate pH 5.0, 10% Polyethylene glycol 10000 |
