7XI5

Anti-CRISPR-associated Aca10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2021-08-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	109.920, 109.920, 60.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.270 - 1.760
R-factor	0.2088
Rwork	0.208
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j9i
RMSD bond length	0.007
RMSD bond angle	0.958
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.270	1.823
High resolution limit [Å]	1.760	1.760
Number of reflections	37421	3674
<I/σ(I)>	25.08
Completeness [%]	99.9
Redundancy	26.4
CC(1/2)	1.000	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	12% (v/v) 2-propanol, 0.1M Imidazole pH7.8